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Lutz Lab

Point-of-care diagnostics for

global health

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  • Home
  • Research
  • Research Positions
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  • People
  • Former members
  • News
  • Contact us
    • Welcome to Lutz Lab

      Simple phenomena + clinical need + real-world constraints

      As bioengineers we seek to apply scientific understanding to develop technology for improving human health. Our work is defined by manipulation of simple physical and biochemical phenomena to create devices for unmet clinical needs, and attention to real-world constraints improves the chances that our work will impact patients. The interplay between science, technology, clinical need, and real-world constraints creates a puzzle that requires a disciplined form of creativity and invention.

    • Positions available with Lutz Labs

      The Lutz group is recruiting postdoctoral researchers and research engineers to develop novel point-of-care diagnostics for global health and home testing. We have multiple projects in areas of HIV, respiratory infection (including Covid-19), and other infectious diseases. We are looking for strong thinkers who can contribute in the following areas:

      • Point of care device engineering (mechanical prototyping, optical detection, low-cost electrical design) 
      • Development of novel assays (RNA/DNA detection, nucleic acid amplification) 

      Please send your CV to Dr. Lutz with the subject “Lutz Lab Hiring 2021”

      Please send your CV to Dr. Lutz with the subject “Lutz Lab Hiring 2021”

    • Publications

      Representative peer-reviewed journal publications

      Ultrasensitive hybridization capture: Reliable detection of <1 copy/mL short cell-free DNA from large-volume urine samples

      Amy Oreskovic, Barry R Lutz. PLOS ONE 16(2): e0247851 (2021)

      Diagnosing pulmonary tuberculosis using sequence-specific purification of urine cell-free DNA

      Amy Oreskovic, Nuttada Panpradist, Diana Marangu, M. William Ngwane, Zanele P. Magcaba, Sindiswa Ngcobo, Zinhle Ngcobo, David J. Horne, Douglas P.K. Wilson, Adrienne E. Shapiro, Paul K. Drain, Barry R. Lutz. medRxiv (2021)

      Near point-of-care, point-mutation test to detect drug resistance in HIV-1: a validation study in a Mexican cohort

      Nuttada Panpradist 1,2 *, Ingrid A. Beck 3 *, Parker S. Ruth 1,4 , Santiago Avilla-Rios 5, Claudia Garcia-Morales 5 , Maribel Soto-Nava 5 , Daniela Tapia-Trejo 5 , Margarita Matias-Florentino 5, Hector E. Paz-Juarez 5, Silvia del Arenal-Sanchez 5, Gustavo Reyes-Teran 5 , Barry R. Lutz 1§ , Lisa M Frenkel 3,6. AIDS (in press).

      The Seattle Flu Study: a multi-arm community-based prospective study protocol for assessing influenza prevalence, transmission, and genomic epidemiology

      Helen Y Chu, Michael Boeckh, Janet A Englund, Michael Famulare, Barry R Lutz, Deborah A Nickerson, Mark J Rieder, Lea M Starita, Amanda Adler, Elisabeth Brandstetter, Chris D Frazar, Peter D Han, Reena K Gularti, James Hadfield, Michael L Jackson, Anahita Kiavand, Louise E Kimball, Kirsten Lacombe, Jennifer Logue, Victoria Lyon, Kira L Newman, Thomas R Sibley, Monica L Zigman Suschsland, Caitlin Wolf, Jay Shendure, Trevor Bedford. medRxiv (repository during peer review).

      OLA-Simple: A software-guided HIV-1 drug resistance test for low-resource laboratories

      Nuttada Panpradist, Ingrid A Beck, Justin Vrana, Nikki Higa, David McIntyre, Parker S. Ruth, Isaac So, Enos C. Kline, Ruth Kanthula, Annie Wong-On-Wing, Jonathan Lim, Daisy Ko, Ross Milne, Theresa Rossouw, Ute D Feucht, Michael Chung, Gonzague Jourdain, Nicole Ngo-Giang-Huong, Laddawan Laomanit, Jaime Soria, James Lai, Eric D. Klavins, Lisa M. Frenkel, Barry R. Lutz. EBioMedicine (published by The Lancet) 50, 34-44, 2 (2019). Editorial discussion by Marc Noguera-Julian (HIV drug resistance testing – the quest for point-of-care”).

      Analytical Comparison of Methods for Extraction of Short Cell-Free DNA from Urine

      A. Oreskovic, ND Brault, N. Panpradist, JJ Lai, BR Lutz. The Journal of Molecular Diagnostics 21 (6), 1067-1078 2 2019

      1775. A Community-wide Study to Evaluate the Accuracy of Self-testing for Influenza: Works in Progress

      Matthew Thompson, Monica L. Zigman Suchsland, Victoria Lyon, Enos Kline, ShiChu Huang, Helen Y. Chu, Mark Rieder, Lea Starita, Jeris Bosua, Barry R. Lutz. Open Forum Infectious Diseases, 6 Supplement 2, S654-S654 (2019)

      Threshold-Based Quantification in a Multiline Lateral Flow Assay via Computationally Designed Capture Efficiency

      DJ Gasperino, D Leon, B Lutz, DM Cate, KP Nichols, D Bell, BH Weigl. Analytical chemistry 90 (11), 6643-6650 (2018)

      Frequency characterization of flow magnitude and phase in resonant microfluidic circuits

       

      Rahil Jain, Robert B. Darling and Barry Lutz Analytical Methods (2017)

      Featured on the Front Cover and as HOT ARTICLE

       

      Frequency tuning allows flow direction control in microfluidic networks with passive features

      Rahil Jain and Barry Lutz Lab on a Chip (2017)

      Featured on the Front Cover and as HOT ARTICLE

      Flow control using audio tones in resonant microfluidic networks: towards cell-phone controlled lab-on-a-chip devices

      Reid Phillips, Rahil Jain, Yoni Browning, Rachana Shah, Peter Kauffman, Doan Dinh and Barry Lutz. Lab on a Chip (2016)

      Highlighted as Editor's Choice in STM

      Simplified paper format for detecting HIV drug resistance in clinical specimens by oligonucleotide ligation

      Nuttada Panpradist, Ingrid Beck, Michael Chung, James Kiarie, Lisa Frenkel and Barry Lutz. PLoS ONE (2016)

      Isothermal Strand Displacement Amplification (iSDA): A Rapid and Sensitive Method of Nucleic Acid Amplification for Point-of-Care Diagnosis

      Bhushan Toley, Isabela Covelli, Yevgeniy Belousov, Sujatha Ramachandran, Enos Kline, Noah Scarr, Nic Vermeulen, Walt Mahoney, Barry Lutz and Paul Yager. Analyst (2015)

      One-step purification and concentration of DNA in porous membranes for point-of-care applications

      Samantha Byrnes, Josh Bishop, Lisa Lafleur, Josh Buser, Barry Lutz and Paul Yager Lab on a Chip (2015)

      A versatile valving toolkit for automating fluidic operations in paper microfluidic devices

      Bhushan Toley, Jessica Wang, Mayuri Gupta, Josh Buser, Lisa Lafleur, Barry Lutz, Elain Fu and Paul Yager Lab on a Chip (2015)

      Swab Sample Transfer for Point-Of-Care Diagnostics: Characterization of Swab Types and Manual Agitation Methods

      Nuttada Panpradist, Bhushan Toley, Xio Zhang, Sam Byrnes, Josh Buser, Janet Englund and Barry Lutz PloS ONE (2014)

      Long-term dry storage of an enzyme-based reagent system for ELISA in point-of-care devices

      Sujatha Ramachandran, Elaine Fu, Barry Lutz and Paul Yager. Analyst (2014)

      Dissolvable fluidic time delays for programming multi-step assays in instrument-free paper diagnostics

      Barry Lutz, Tinny Liang, Elaine Fu, Sujatha Ramachandran, Peter Kauffman and Paul Yager. Lab on a Chip (2013)

      Subject of a “Viewpoints” article by George Whitesides, Lab on a Chip (2013), DOI: 10.1039/C3LC90066e. Also featured in Chemistry World, May 30, 2013, ”Microfluidic sugar paper” with quotes from George Whitesides

      A Rapid, Multiplexed, High-Throughput Flow-Through Membrane Immunoassay: A Convenient Alternative to ELISA

      Sujatha Ramachandran, Mitra Singhal, Katherine McKenzie, Jennifer L. Osborn, Amit Arjyal, Sabina Dongol, Stephen Baker, Buddha Basnyat, Jeremy Farrar, Christiane Dolecek, Gonzalo J. Domingo, Paul Yager and Barry Lutz. Diagnostics, Special Issue: Feature Papers (2013),

      New and Improved Ways to Treat Hydrocephalus: pursuit of a smart shunt

      Barry Lutz, Pranav Venkataraman and Sam Browd. Surgical Neurology International (2013)

      Progress toward multiplexed sample-to-result detection in low resource settings using microfluidic immunoassay cards

      Lisa Lafleur, Dean Stevens, Katherine McKenzie, Sujatha Ramachandran, Paolo Spicar-Mihalic, Mitra Singhal, Amit Arjyal, Jennifer Osborn, Peter Kauffman, Paul Yager and  Barry Lutz. Lab on a Chip (2012)

      Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper

      Barry Lutz, Philip Trinh, Cameron Ball, Elaine Fu and Paul Yager. Lab on a Chip (2011)

      Enhanced Sensitivity of Lateral Flow Tests Using a Two-Dimensional Paper Network Format

      Elain Fu, Tinny Liang, Jared Houghtaling, Sujatha Ramachandran, Stephen A. Ramsey, Barry Lutz and Paul Yager. Analytical Chemistry (2011)

      Transport in two-dimensional paper networks

      Elain Fu, Stephen Ramsey, Peter Kauffman, Barry Lutz and Paul Yager Microfluidics and Nanofluidics (2011)

      Microfluidics without pumps: re-inventing the T-sensor & H-filter in paper networks

      Jennifer Osborn, Barry Lutz, Elain Fu, Peter Kauffman, Dean Y. Stevensa and Paul Yager Lab on a Chip (2010)

      Chemical signal amplification in two-dimensional paper networks

      Elain Fu, Peter Kauffman, Barry Lutz, Paul Yager. Sensors & Actuators B (2010)

      Visualization and measurement of flow in two-dimensional paper networks

      Peter Kauffman, Elain Fu, Barry Lutz and Paul Yager. Lab on a Chip (2010)

      Controlled reagent transport in disposable 2D paper networks

      Elain Fu, Barry Lutz, Peter Kauffman and Paul Yager. Lab on a Chip (2010)

      Rapid protein depletion from complex samples using a bead-based microfluidic device for the point of care

      Katherine McKenzie, Lisa Lafleur, Barry Lutz and  Paul Yager. Lab on a Chip (2009)

    • People

      Current lab members

      Barry Lutz

      Assistant Professor

      Ph.D. Chemical Engineering

      Phone: (206) 685-9891
      Office: Foege N530N

      blutz@uw.edu
      ResearchGate Profile

      Email

      Enos Kline

      Research Scientist

      DARPA MAD NAAT project

      klinee@uw.edu
      ResearchGate Profile

      Email

      Bob Atkinson

      BioE Ph.D. Student

      Point-of-care multiplexable nucleic acid assay for STDs

      bob@theatkinsons.org

      Email

      Nuttada Panpradist

      BioE Ph.D. Student

      OLA-Simple – HIV Drug Resistance Test

      LinkedIn Profile
      Google Scholar Profile

      ResearchGate Profile

      nuttadap@uw.edu

      Email

      Amy Oreskovic

      BioE Ph.D. student
      NSF Graduate Fellow

      TB diagnosis from urine cell-free DN

      LinkedIn

      Google Scholar Profile

      akoresk@uw.edu

      Email

      Ian Hull

      BioE Ph.D. Student

      Polymerase engineering for direct nucleic acid diagnostics

      LinkedIn

      ianhull@uw.edu

      Email

      Daniel Leon

      Research Engineer

      CrossLife TARA detection projects
      TB-drug adherence at-home monitoring project

      dleon@uw.edu

      Email

      Qin Wang

      BioE Ph.D. Student

      HIV Self-test
      LinkedIn Profile
      Google Scholar Profile

      qw42@uw.edu

      Email
    • Former Members



      Sam Stewart , Research Scientist, OLA-Simple – HIV Drug Resistance Test

      Rahil Jain, EEPh.D. Candidate

      Annapurni Sriram, Undergraduate Student (OLA-Simple - HIV Drug Resistance Test)

      Rosemichelle Marzan, Undergraduate Student, (Collaboration Project with Global Good)

      David McIntyre, Undergraduate Student (OLA-Simple - HIV Drug Resistance Test)

      Jonathan Lim, Undergraduate Student (Point-of-care pulmonary TB detection using urine)

      Zachary McNulty, Undergraduate Student (Point-of-care pulmonary TB detection using urine)

       

      2017 Graduates

      Annie Wing-on-Wong, BioE Senior Capstone Student (OLA-Simple), Currently at Seattle Children's Hospital

      Bella Spielman, BioE Senior Capstone Student (OLA-Simple), Currently at Accenture

      Nikka Higga, BioE Senior Capstone Student (OLA-Simple), Currently at Seattle Children's Hospital

       

      2016 Graduates

      Doan Dinh, BioE Senior Capstone Student (Lab on a phone call project), Currently at Physio Control

      Ian Andrews, BioE Senior Capstone Student (OLA-Simple), Currently at MIT

       

      2015 and before

      Shivani Dharmaraja, Masters Student (Point-of-Care Diagnostics for HIV)

      Madhav Murthy, Undergraduate Researcher (Point-of-Care Diagnostics for HIV)

      Ricky Liu, BioE Senior Capstone Student (Capillary-driven Point-of-Care Diagnostics, collaboration with Deok-Ho Kim)

      Pranav Venkataraman, BioE Senior Capstone Student (Smart Shunt Project)

      Marie Eberlein, BioE Senior Capstone Student (AC Fluidics Project)

      Chenyi Wang, BioE Senior Capstone Student (Smart Shunt Project)

      Bibianna Cha, Research Engineer (Smart Shunt Project)

      Joel Relethford, Research Engineer (Smart Shunt Project)

      James Wong. BioE Senior Capstone Student (AC Fluidics Project, Protein Printing)

      Damon Cassisi. Research Engineer (Smart Shunt Project)

      Rachana Shah. Graduate Student, MS 2010 (AC Fluidics Project)

      Yoni Browning. Undergraduate Student (AC Fluidics Project)

      Reid Philips. BioE Senior Capstone Student (AC Fluidics Project)

    • News

      Updates from the inside and around the lab

      2021

      02/01
      Nuttada's paper from 2014 on swabs for point-of-care testing is getting a lot of attention lately. She characterized the release of samples from different nasal swab types, including the effect of manual agitation and a novel design for flow through swabs - very relevant today for Covid testing. She is working on a follow-up paper - coming soon!


      2020

      03/27
      The Lutz group is recruiting for postdoctoral researchers and research engineers to develop novel point of care diagnostics for global health and home testing. We have multiple projects in areas of HIV, respiratory infection (including Covid-19), and other infectious diseases. We are looking for strong thinkers who can contribute in the following areas:

      • Point of care device engineering (mechanical prototyping, optical detection, low-cost electrical design) 
      • Development of novel assays (RNA/DNA detection, nucleic acid amplification) 

      Please send your CV to Dr. Lutz with the subject “Lutz Lab Hiring 2020”

      2018

      03/08

      Rahil passed his defense. Congratulations!

       

      03/01

      OLA Simple won the 2nd place $10k award at the annual Health Innovation Challenge

      https://www.geekwire.com/2018/student-entrepreneurs-tackle-genetic-testing-clinical-trials-health-startup-competition/

      2017

      09/09

      Dr. Lutz traveled to Nepal as part of a Bioengineering department group to make ties to Kathmandu University.

       

      09/01

      Rahil's paper on the flow magnitude and phase measurement in resonant devices is published and selected to be featured on the Front Cover of the Journal Analytical Methods.

       

      http://pubs.rsc.org/en/content/articlelanding/2017/ay/c7ay01699a#!divAbstract

       

      08/22 - 09/15

      Rahil got the Amazon Catalyst grant ($50k) and the GIX Innovation Award ($20k) to further work on commercializing his side projects.

       

      08/15

      Dr. Nathan White (UW, Emergency medicine) and Dr. Lutz submitted a new collaboration grant to work on a smartphone-powered thromboelastography device based on the resonant microfluidics project.

       

      07/14

      Nuttada received the prestigious 2017 UW Medicine Bud Tribble and Susan Barnes Graduate Discovery Fellowship. To learn more: https://bioe.uw.edu/nuttada-panpradist-receives-graduate-discovery-fellowship/

       

      05/01

      Rahil's paper featured on the Front Cover of the Journal Lab on a Chip Volume 17 Number 9

      http://pubs.rsc.org/en/content/articlelanding/2017/lc/c7lc90043k#!divAbstract

      News article - https://bioe.uw.edu/ph-d-student-rahil-jain-investigates-smart-solutions-for-diagnostics-home-electronics/

       

      04/27

      Nuttada passed her Generals! Congratulations!

       

      03/06

      Dr. Lutz traveled to Bangladesh to experience the global health needs first-hand.

       

      02/06

      Nuttada and her OLA-Simple team were featured on KIRO NEWS

      https://www.engr.washington.edu/news/panpradist

       

      01/28

      Rahil passed his Generals. Congratulations!

      2016

      08/31

      Congratulation to Nuttada and Rahil for being selected as 10 finalists around the nation for the APF Student Technology Prize for Primary Healthcare. The competition, administered by Massachusetts General Hospital's Ambulatory Practice of the Future (APF) and sponsored by the Gelfand Family Charitable Trust, encourages and supports engineering students' efforts to develop innovative, clinically-relevant primary health care technology concepts.

       

      06/10

      Congratulations to Doan and Ian on graduating. Ian made it to coveted Husky 100 and is heading to MIT for graduate school. Doan will be working with Physio Control in Redmond WA. Good luck to you both.

       

      05/12 

      Congratulations to Nuttada and Rahil to make it final round of APF Student Technology Prize. Both are independently getting $10k to further their research. Final proposals are due Aug 29.

       

      03/11

      Congratulations to Annie on receiving Mary Gates Research Scholarship!

       

      02/12

      Nuttada and Dr. James Lai (our collaborator on the OLA-Simple Project) wrote a chapter on point-of-care diagnostics as part of the book titled "Biomaterials Nanoarchitectonics" edited by Mitsuhiro Ebara. The book has just been published and now available at Elsevier website and can be downloaded via the UW library.

       

      02/01

      Bioengineer sophomore Jonathan Lim joined the Lutz Lab and will work on the TB project. Welcome Jonathan!!!

       

      01/11

      Nuttada's manuscript in PLoS ONE! "Simplified paper format for detecting HIV drug resistance in clinical specimens by oligonucleotide ligation" - see publications

       

      02/20 - 02/21

      Nuttada, Annie, Ian and Nikki are presenting at the XXV International HIV Drug Resistance Workshop in Boston, MA (a closed workshop, with a limited attendance of 200 delegates). Stop by and check out their poster presentations in the New Resistance Technologies and Interpretations Session!

       

      01/20

      Nuttada is co-lecturing with Dr. Sylvia LaCourse in the GH590 Global WACh BioEngineering Seminar on "Opportunities for Point-of-care TB Diagnostics" Come by at 4:30 - 5:30 PM at EEB 037 in the Electrical Engineering Building!

       

      01/04 - 03/11

      Dr. Lutz is teaching the Global Health Technology: Molecular Diagnostics!

       

      01/04 - 03/11

      Nuttada is TAing for BIOEN 531 Proposal Writing by Dr. Marta Scatena - one of the three Bioengineering Graduate Core classes! She is teaching a class on oral presentation and grant reviewing process on the Feb 8th, 2015.

      2016

      08/31

      Congratulation to Nuttada and Rahil for being selected as 10 finalists around the nation for the APF Student Technology Prize for Primary Healthcare. The competition, administered by Massachusetts General Hospital's Ambulatory Practice of the Future (APF) and sponsored by the Gelfand Family Charitable Trust, encourages and supports engineering students' efforts to develop innovative, clinically-relevant primary health care technology concepts.

       

      06/10

      Congratulations to Doan and Ian on graduating. Ian made it to coveted Husky 100 and is heading to MIT for graduate school. Doan will be working with Physio Control in Redmond WA. Good luck to you both.

       

      05/12 

      Congratulations to Nuttada and Rahil to make it final round of APF Student Technology Prize. Both are independently getting $10k to further their research. Final proposals are due Aug 29.

       

      03/11

      Congratulations to Annie on receiving Mary Gates Research Scholarship!

       

      02/12

      Nuttada and Dr. James Lai (our collaborator on the OLA-Simple Project) wrote a chapter on point-of-care diagnostics as part of the book titled "Biomaterials Nanoarchitectonics" edited by Mitsuhiro Ebara. The book has just been published and now available at Elsevier website and can be downloaded via the UW library.

       

      02/01

      Bioengineer sophomore Jonathan Lim joined the Lutz Lab and will work on the TB project. Welcome Jonathan!!!

       

      01/11

      Nuttada's manuscript in PLoS ONE! "Simplified paper format for detecting HIV drug resistance in clinical specimens by oligonucleotide ligation" - see publications

       

      02/20 - 02/21

      Nuttada, Annie, Ian and Nikki are presenting at the XXV International HIV Drug Resistance Workshop in Boston, MA (a closed workshop, with a limited attendance of 200 delegates). Stop by and check out their poster presentations in the New Resistance Technologies and Interpretations Session!

       

      01/20

      Nuttada is co-lecturing with Dr. Sylvia LaCourse in the GH590 Global WACh BioEngineering Seminar on "Opportunities for Point-of-care TB Diagnostics" Come by at 4:30 - 5:30 PM at EEB 037 in the Electrical Engineering Building!

       

      01/04 - 03/11

      Dr. Lutz is teaching the Global Health Technology: Molecular Diagnostics!

       

      01/04 - 03/11

      Nuttada is TAing for BIOEN 531 Proposal Writing by Dr. Marta Scatena - one of the three Bioengineering Graduate Core classes! She is teaching a class on oral presentation and grant reviewing process on the Feb 8th, 2015.

    • Contact us

      Inquiries or correspondence should be sent to:

       

      Barry Lutz

      blutz@uw.edu

      University of Washington

      Department of Bioengineering

      3720 15th Avenue NE

      Box 355061

      Seattle WA 98195

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    University of Washington, Seattle

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