Point-of-care diagnostics for
Welcome to Lutz Lab
Simple phenomena + clinical need + real-world constraints
As bioengineers we seek to apply scientific understanding to develop technology for improving human health. Our work is defined by manipulation of simple physical and biochemical phenomena to create devices for unmet clinical needs, and attention to real-world constraints improves the chances that our work will impact patients. The interplay between science, technology, clinical need, and real-world constraints creates a puzzle that requires a disciplined form of creativity and invention.
Nuttada Panpradist Receives UW College of Engineering Award
Read about the SARS-CoV-2 technology developed from Lutz lab @uwbioe MedRxiv. A novel four-plexed RT-LAMP test kit operated by health workers can provide faster and more sensitive results than lab tests. https://www.medrxiv.org/content/10.1101/2021.08.12.21261875v1.full.pdf+html
Lutz Lab's Nuttada Panpradist will join an expert panel at today's WE-REACH fireside chat to discuss biomedical business models, budgets, and funding sources. https://www.seattleangelconference.com
Other experts who will be on the panel are:
• John Sechrest, CEO, Seattle Angel Conference
• Elizabeth Cross Nichol, APIS Health Angels, Seattle
• Minda Brusse, First Row Partners, Seattle
Previously Lutz Lab received funding from WE-REACH to support the commercialization of the point-of-care COVID-19 test.
June 7Congratulations to Nuttada for being recognized for outstanding contributions to UW Bioengineering research! The UW College of Engineering Awards acknowledged the extraordinary efforts of the college’s teaching and research assistants, staff and faculty members.Nuttada Panpradist’s impressive academic career includes 13 publications, 17 conference proceedings, and four patent applications. Her simple point-of-care tests detect HIV genetic mutations to help doctors prescribe non-resistant drugs. She designed rapid lab tests for COVID-19 and devices to ensure patients are taking their TB medication. She has collaborated with various researchers on COVID-19 projects such as developing new methods to detect SARS-CoV-2 in Seattle to validating point-of-care tests in pediatric clinics. As a mentor, Panpradist connects undergraduate students with research projects that match their interests. All her students have received research scholarships and are named as co-authors and co-inventors of patents. “Nuttada has gone above and beyond to foster our new collaboration; beyond her scientific acumen, she is an attentive and professional partner, mature beyond her years, conscientious, flexible, able to balance multiple responsibilities, and has an impressive work ethic.”05/26Amy passed her Ph.D. defense, titled "Improving diagnosis of tuberculosis from urine cell-free DNA." Congratulations Amy!"02/01Nuttada's paper from 2014 on swabs for point-of-care testing is getting a lot of attention lately. She characterized the release of samples from different nasal swab types, including the effect of manual agitation and a novel design for flow through swabs - very relevant today for Covid testing. She is working on a follow-up paper - coming soon!
Positions available with Lutz Labs
The Lutz group is recruiting postdoctoral researchers and research engineers to develop novel point-of-care diagnostics for global health and home testing. We have multiple projects in areas of HIV, respiratory infection (including Covid-19), and other infectious diseases. We are looking for strong thinkers who can contribute in the following areas:
Please send your CV to Dr. Lutz with the subject “Lutz Lab Hiring 2021”
Ultrasensitive hybridization capture: Reliable detection of <1 copy/mL short cell-free DNA from large-volume urine samples
Amy Oreskovic, Barry R Lutz. PLOS ONE 16(2): e0247851 (2021)
Diagnosing pulmonary tuberculosis using sequence-specific purification of urine cell-free DNA
Amy Oreskovic, Nuttada Panpradist, Diana Marangu, M. William Ngwane, Zanele P. Magcaba, Sindiswa Ngcobo, Zinhle Ngcobo, David J. Horne, Douglas P.K. Wilson, Adrienne E. Shapiro, Paul K. Drain, Barry R. Lutz. Journal of Clinical Microbiology 59(8):e00074-21 (2021).
Near point-of-care, point-mutation test to detect drug resistance in HIV-1: a validation study in a Mexican cohort
Nuttada Panpradist 1,2 *, Ingrid A. Beck 3 *, Parker S. Ruth 1,4 , Santiago Avilla-Rios 5, Claudia Garcia-Morales 5 , Maribel Soto-Nava 5 , Daniela Tapia-Trejo 5 , Margarita Matias-Florentino 5, Hector E. Paz-Juarez 5, Silvia del Arenal-Sanchez 5, Gustavo Reyes-Teran 5 , Barry R. Lutz 1§ , Lisa M Frenkel 3,6. AIDS (in press).
The Seattle Flu Study: a multi-arm community-based prospective study protocol for assessing influenza prevalence, transmission, and genomic epidemiology
Helen Y Chu, Michael Boeckh, Janet A Englund, Michael Famulare, Barry R Lutz, Deborah A Nickerson, Mark J Rieder, Lea M Starita, Amanda Adler, Elisabeth Brandstetter, Chris D Frazar, Peter D Han, Reena K Gularti, James Hadfield, Michael L Jackson, Anahita Kiavand, Louise E Kimball, Kirsten Lacombe, Jennifer Logue, Victoria Lyon, Kira L Newman, Thomas R Sibley, Monica L Zigman Suschsland, Caitlin Wolf, Jay Shendure, Trevor Bedford. medRxiv (repository during peer review).
OLA-Simple: A software-guided HIV-1 drug resistance test for low-resource laboratories
Nuttada Panpradist, Ingrid A Beck, Justin Vrana, Nikki Higa, David McIntyre, Parker S. Ruth, Isaac So, Enos C. Kline, Ruth Kanthula, Annie Wong-On-Wing, Jonathan Lim, Daisy Ko, Ross Milne, Theresa Rossouw, Ute D Feucht, Michael Chung, Gonzague Jourdain, Nicole Ngo-Giang-Huong, Laddawan Laomanit, Jaime Soria, James Lai, Eric D. Klavins, Lisa M. Frenkel, Barry R. Lutz. EBioMedicine (published by The Lancet) 50, 34-44, 2 (2019). Editorial discussion by Marc Noguera-Julian (HIV drug resistance testing – the quest for point-of-care”).
Analytical Comparison of Methods for Extraction of Short Cell-Free DNA from Urine
A. Oreskovic, ND Brault, N. Panpradist, JJ Lai, BR Lutz. The Journal of Molecular Diagnostics 21 (6), 1067-1078 2 2019
1775. A Community-wide Study to Evaluate the Accuracy of Self-testing for Influenza: Works in Progress
Matthew Thompson, Monica L. Zigman Suchsland, Victoria Lyon, Enos Kline, ShiChu Huang, Helen Y. Chu, Mark Rieder, Lea Starita, Jeris Bosua, Barry R. Lutz. Open Forum Infectious Diseases, 6 Supplement 2, S654-S654 (2019)
Threshold-Based Quantification in a Multiline Lateral Flow Assay via Computationally Designed Capture Efficiency
DJ Gasperino, D Leon, B Lutz, DM Cate, KP Nichols, D Bell, BH Weigl. Analytical chemistry 90 (11), 6643-6650 (2018)
Frequency characterization of flow magnitude and phase in resonant microfluidic circuits
Rahil Jain, Robert B. Darling and Barry Lutz Analytical Methods (2017)
Featured on the Front Cover and as HOT ARTICLE
Frequency tuning allows flow direction control in microfluidic networks with passive features
Rahil Jain and Barry Lutz Lab on a Chip (2017)
Flow control using audio tones in resonant microfluidic networks: towards cell-phone controlled lab-on-a-chip devices
Reid Phillips, Rahil Jain, Yoni Browning, Rachana Shah, Peter Kauffman, Doan Dinh and Barry Lutz. Lab on a Chip (2016)
Highlighted as Editor's Choice in STM
Simplified paper format for detecting HIV drug resistance in clinical specimens by oligonucleotide ligation
Nuttada Panpradist, Ingrid Beck, Michael Chung, James Kiarie, Lisa Frenkel and Barry Lutz. PLoS ONE (2016)
Isothermal Strand Displacement Amplification (iSDA): A Rapid and Sensitive Method of Nucleic Acid Amplification for Point-of-Care Diagnosis
Bhushan Toley, Isabela Covelli, Yevgeniy Belousov, Sujatha Ramachandran, Enos Kline, Noah Scarr, Nic Vermeulen, Walt Mahoney, Barry Lutz and Paul Yager. Analyst (2015)
One-step purification and concentration of DNA in porous membranes for point-of-care applications
Samantha Byrnes, Josh Bishop, Lisa Lafleur, Josh Buser, Barry Lutz and Paul Yager Lab on a Chip (2015)
A versatile valving toolkit for automating fluidic operations in paper microfluidic devices
Bhushan Toley, Jessica Wang, Mayuri Gupta, Josh Buser, Lisa Lafleur, Barry Lutz, Elain Fu and Paul Yager Lab on a Chip (2015)
Swab Sample Transfer for Point-Of-Care Diagnostics: Characterization of Swab Types and Manual Agitation Methods
Nuttada Panpradist, Bhushan Toley, Xio Zhang, Sam Byrnes, Josh Buser, Janet Englund and Barry Lutz PloS ONE (2014)
Long-term dry storage of an enzyme-based reagent system for ELISA in point-of-care devices
Sujatha Ramachandran, Elaine Fu, Barry Lutz and Paul Yager. Analyst (2014)
Dissolvable fluidic time delays for programming multi-step assays in instrument-free paper diagnostics
Barry Lutz, Tinny Liang, Elaine Fu, Sujatha Ramachandran, Peter Kauffman and Paul Yager. Lab on a Chip (2013)
Subject of a “Viewpoints” article by George Whitesides, Lab on a Chip (2013), DOI: 10.1039/C3LC90066e. Also featured in Chemistry World, May 30, 2013, ”Microfluidic sugar paper” with quotes from George Whitesides
A Rapid, Multiplexed, High-Throughput Flow-Through Membrane Immunoassay: A Convenient Alternative to ELISA
Sujatha Ramachandran, Mitra Singhal, Katherine McKenzie, Jennifer L. Osborn, Amit Arjyal, Sabina Dongol, Stephen Baker, Buddha Basnyat, Jeremy Farrar, Christiane Dolecek, Gonzalo J. Domingo, Paul Yager and Barry Lutz. Diagnostics, Special Issue: Feature Papers (2013),
New and Improved Ways to Treat Hydrocephalus: pursuit of a smart shunt
Barry Lutz, Pranav Venkataraman and Sam Browd. Surgical Neurology International (2013)
Progress toward multiplexed sample-to-result detection in low resource settings using microfluidic immunoassay cards
Lisa Lafleur, Dean Stevens, Katherine McKenzie, Sujatha Ramachandran, Paolo Spicar-Mihalic, Mitra Singhal, Amit Arjyal, Jennifer Osborn, Peter Kauffman, Paul Yager and Barry Lutz. Lab on a Chip (2012)
Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper
Barry Lutz, Philip Trinh, Cameron Ball, Elaine Fu and Paul Yager. Lab on a Chip (2011)
Enhanced Sensitivity of Lateral Flow Tests Using a Two-Dimensional Paper Network Format
Elain Fu, Tinny Liang, Jared Houghtaling, Sujatha Ramachandran, Stephen A. Ramsey, Barry Lutz and Paul Yager. Analytical Chemistry (2011)
Transport in two-dimensional paper networks
Elain Fu, Stephen Ramsey, Peter Kauffman, Barry Lutz and Paul Yager Microfluidics and Nanofluidics (2011)
Microfluidics without pumps: re-inventing the T-sensor & H-filter in paper networks
Jennifer Osborn, Barry Lutz, Elain Fu, Peter Kauffman, Dean Y. Stevensa and Paul Yager Lab on a Chip (2010)
Chemical signal amplification in two-dimensional paper networks
Elain Fu, Peter Kauffman, Barry Lutz, Paul Yager. Sensors & Actuators B (2010)
Visualization and measurement of flow in two-dimensional paper networks
Peter Kauffman, Elain Fu, Barry Lutz and Paul Yager. Lab on a Chip (2010)
Controlled reagent transport in disposable 2D paper networks
Elain Fu, Barry Lutz, Peter Kauffman and Paul Yager. Lab on a Chip (2010)
Rapid protein depletion from complex samples using a bead-based microfluidic device for the point of care
Katherine McKenzie, Lisa Lafleur, Barry Lutz and Paul Yager. Lab on a Chip (2009)
Current lab members
Ph.D. Chemical Engineering
Phone: (206) 685-9891Office: Foege N530N
DARPA MAD NAAT project
BioE Ph.D. Student
Point-of-care multiplexable nucleic acid assay for STDs
OLA-Simple – HIV Drug Resistance Test
LinkedIn ProfileGoogle Scholar Profile
Polymerase engineering for direct nucleic acid diagnostics
CrossLife TARA detection projectsTB-drug adherence at-home monitoring project
HIV Self-testLinkedIn ProfileGoogle Scholar Profile
Amy Oreskovic, Ph.D. student, (TB diagnosis from urine cell-free DNA)
Sam Stewart, Research Scientist, OLA-Simple – HIV Drug Resistance Test
Rahil Jain, EEPh.D. Candidate
Annapurni Sriram, Undergraduate Student (OLA-Simple - HIV Drug Resistance Test)
Rosemichelle Marzan, Undergraduate Student, (Collaboration Project with Global Good)
David McIntyre, Undergraduate Student (OLA-Simple - HIV Drug Resistance Test)
Jonathan Lim, Undergraduate Student (Point-of-care pulmonary TB detection using urine)
Zachary McNulty, Undergraduate Student (Point-of-care pulmonary TB detection using urine)
Annie Wing-on-Wong, BioE Senior Capstone Student (OLA-Simple), Currently at Seattle Children's Hospital
Bella Spielman, BioE Senior Capstone Student (OLA-Simple), Currently at Accenture
Nikka Higga, BioE Senior Capstone Student (OLA-Simple), Currently at Seattle Children's Hospital
Doan Dinh, BioE Senior Capstone Student (Lab on a phone call project), Currently at Physio Control
Ian Andrews, BioE Senior Capstone Student (OLA-Simple), Currently at MIT
2015 and before
Shivani Dharmaraja, Masters Student (Point-of-Care Diagnostics for HIV)
Madhav Murthy, Undergraduate Researcher (Point-of-Care Diagnostics for HIV)
Ricky Liu, BioE Senior Capstone Student (Capillary-driven Point-of-Care Diagnostics, collaboration with Deok-Ho Kim)
Pranav Venkataraman, BioE Senior Capstone Student (Smart Shunt Project)
Marie Eberlein, BioE Senior Capstone Student (AC Fluidics Project)
Chenyi Wang, BioE Senior Capstone Student (Smart Shunt Project)
Bibianna Cha, Research Engineer (Smart Shunt Project)
Joel Relethford, Research Engineer (Smart Shunt Project)
James Wong. BioE Senior Capstone Student (AC Fluidics Project, Protein Printing)
Damon Cassisi. Research Engineer (Smart Shunt Project)
Rachana Shah. Graduate Student, MS 2010 (AC Fluidics Project)
Yoni Browning. Undergraduate Student (AC Fluidics Project)
Reid Philips. BioE Senior Capstone Student (AC Fluidics Project)
03/27The Lutz group is recruiting for postdoctoral researchers and research engineers to develop novel point of care diagnostics for global health and home testing. We have multiple projects in areas of HIV, respiratory infection (including Covid-19), and other infectious diseases. We are looking for strong thinkers who can contribute in the following areas:
Please send your CV to Dr. Lutz with the subject “Lutz Lab Hiring 2020”
Rahil passed his defense. Congratulations!
OLA Simple won the 2nd place $10k award at the annual Health Innovation Challenge
Dr. Lutz traveled to Nepal as part of a Bioengineering department group to make ties to Kathmandu University.
Rahil's paper on the flow magnitude and phase measurement in resonant devices is published and selected to be featured on the Front Cover of the Journal Analytical Methods.
08/22 - 09/15
Rahil got the Amazon Catalyst grant ($50k) and the GIX Innovation Award ($20k) to further work on commercializing his side projects.
Dr. Nathan White (UW, Emergency medicine) and Dr. Lutz submitted a new collaboration grant to work on a smartphone-powered thromboelastography device based on the resonant microfluidics project.
Nuttada received the prestigious 2017 UW Medicine Bud Tribble and Susan Barnes Graduate Discovery Fellowship. To learn more: https://bioe.uw.edu/nuttada-panpradist-receives-graduate-discovery-fellowship/
Rahil's paper featured on the Front Cover of the Journal Lab on a Chip Volume 17 Number 9
News article - https://bioe.uw.edu/ph-d-student-rahil-jain-investigates-smart-solutions-for-diagnostics-home-electronics/
Nuttada passed her Generals! Congratulations!
Dr. Lutz traveled to Bangladesh to experience the global health needs first-hand.
Nuttada and her OLA-Simple team were featured on KIRO NEWS
Rahil passed his Generals. Congratulations!
Congratulation to Nuttada and Rahil for being selected as 10 finalists around the nation for the APF Student Technology Prize for Primary Healthcare. The competition, administered by Massachusetts General Hospital's Ambulatory Practice of the Future (APF) and sponsored by the Gelfand Family Charitable Trust, encourages and supports engineering students' efforts to develop innovative, clinically-relevant primary health care technology concepts.
Congratulations to Doan and Ian on graduating. Ian made it to coveted Husky 100 and is heading to MIT for graduate school. Doan will be working with Physio Control in Redmond WA. Good luck to you both.
Congratulations to Nuttada and Rahil to make it final round of APF Student Technology Prize. Both are independently getting $10k to further their research. Final proposals are due Aug 29.
Congratulations to Annie on receiving Mary Gates Research Scholarship!
Nuttada and Dr. James Lai (our collaborator on the OLA-Simple Project) wrote a chapter on point-of-care diagnostics as part of the book titled "Biomaterials Nanoarchitectonics" edited by Mitsuhiro Ebara. The book has just been published and now available at Elsevier website and can be downloaded via the UW library.
Bioengineer sophomore Jonathan Lim joined the Lutz Lab and will work on the TB project. Welcome Jonathan!!!
Nuttada's manuscript in PLoS ONE! "Simplified paper format for detecting HIV drug resistance in clinical specimens by oligonucleotide ligation" - see publications
02/20 - 02/21
Nuttada, Annie, Ian and Nikki are presenting at the XXV International HIV Drug Resistance Workshop in Boston, MA (a closed workshop, with a limited attendance of 200 delegates). Stop by and check out their poster presentations in the New Resistance Technologies and Interpretations Session!
Nuttada is co-lecturing with Dr. Sylvia LaCourse in the GH590 Global WACh BioEngineering Seminar on "Opportunities for Point-of-care TB Diagnostics" Come by at 4:30 - 5:30 PM at EEB 037 in the Electrical Engineering Building!
01/04 - 03/11
Dr. Lutz is teaching the Global Health Technology: Molecular Diagnostics!
Nuttada is TAing for BIOEN 531 Proposal Writing by Dr. Marta Scatena - one of the three Bioengineering Graduate Core classes! She is teaching a class on oral presentation and grant reviewing process on the Feb 8th, 2015.
Inquiries or correspondence should be sent to:
University of Washington
Department of Bioengineering
3720 15th Avenue NE
Seattle WA 98195
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University of Washington, Seattle